F2P – Nutrients for Diatoms

Macronutrients (elements)

Micronutrients (elements)

Vitamins (elements)

Cell-Hi F2P is a highly concentrated nutrient premix where 1 kg makes 10,000 L culture medium at F/2 strength.

RECOMMENDATIONS

Batch cultivation : recommended for use at concentrations between 0.1-1 g/L. The ideal nutrient concentration will depend on the expected / observed final yields.
Continuous cultivation : typically included at a rate of 0.1 g/L for continuous production (this concentration gives a 1x Guillard F/2).

STERILISATION

To ensure sterilisation, diluted media can be passed through a 0.2 micron syringe.
Autoclaving of diluted media is also possible however, this may result in some minor changes to chemical composition.

STORAGE

In order to ensure good mixing of micronutrients when using at small scale and for ease of use a large scale, it is recommended that a high concentrate stock is produced.
For Cell-Hi F2P, this stock should be formulated at 1 kg/10 L and used at 1 mL/L for continuous production.

SPECIAL PRECAUTIONS

Diatoms : For increased productivity during diatom production, 30 mg/L of metasilicate should be added to the growth media.